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J Am Vet Med Assoc. 2013 Jan 15;242(2):242-8. doi: 10.2460/javma.242.2.242.

Comparison of pH and refractometry index with calcium concentrations in preparturient mammary gland secretions of mares.

Author information

1
Hidaka Training and Research Center, Japan Racing Association, Hokkaido, Japan. Kenji_Korosue@jra.go.jp

Abstract

OBJECTIVE:

To test the usefulness of measuring pH and refractometry index, compared with measuring calcium carbonate concentration, of preparturient mammary gland secretions for predicting parturition in mares.

DESIGN:

Evaluation study.

ANIMALS:

27 pregnant Thoroughbred mares.

PROCEDURES:

Preparturient mammary gland secretion samples were obtained once or twice daily 10 days prior to foaling until parturition. The samples were analyzed for calcium carbonate concentration with a water hardness kit (151 samples), pH with pH test paper (222 samples), and refractometry index with a Brix refractometer (214 samples). The sensitivity, specificity, and positive and negative predictive values for each test were calculated for evaluation of predicting parturition.

RESULTS:

The PPV within 72 hours and the NPV within 24 hours for calcium carbonate concentration determination (standard value set to 400 μg/g) were 93.8% and 98.3%, respectively. The PPV within 72 hours and the NPV within 24 hours for the pH test (standard value set at 6.4) were 97.9% and 99.4%, respectively. The PPV within 72 hours and the NPV within 24 hours for the Brix test (standard value set to 20%) were 73.2% and 96.5%, respectively.

CONCLUSIONS AND CLINICAL RELEVANCE:

Results suggested that the pH test with the standard value set at a pH of 6.4 would be useful in the management of preparturient mares by predicting when mares are not ready to foal. This was accomplished with equal effectiveness of measuring calcium carbonate concentration with a water hardness kit.

PMID:
23276103
DOI:
10.2460/javma.242.2.242
[Indexed for MEDLINE]

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