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PLoS Genet. 2012;8(12):e1003121. doi: 10.1371/journal.pgen.1003121. Epub 2012 Dec 13.

Reciprocal signaling between the ectoderm and a mesendodermal left-right organizer directs left-right determination in the sea urchin embryo.

Author information

1
UMR 7009 CNRS, Université de Pierre et Marie Curie (Paris 6), Observatoire Océanologique de Villefranche-sur-Mer, Villefranche-sur-Mer, France.

Abstract

During echinoderm development, expression of nodal on the right side plays a crucial role in positioning of the rudiment on the left side, but the mechanisms that restrict nodal expression to the right side are not known. Here we show that establishment of left-right asymmetry in the sea urchin embryo relies on reciprocal signaling between the ectoderm and a left-right organizer located in the endomesoderm. FGF/ERK and BMP2/4 signaling are required to initiate nodal expression in this organizer, while Delta/Notch signaling is required to suppress formation of this organizer on the left side of the archenteron. Furthermore, we report that the H(+)/K(+)-ATPase is critically required in the Notch signaling pathway upstream of the S3 cleavage of Notch. Our results identify several novel players and key early steps responsible for initiation, restriction, and propagation of left-right asymmetry during embryogenesis of a non-chordate deuterostome and uncover a functional link between the H(+)/K(+)-ATPase and the Notch signaling pathway.

PMID:
23271979
PMCID:
PMC3521660
DOI:
10.1371/journal.pgen.1003121
[Indexed for MEDLINE]
Free PMC Article

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