Format

Send to

Choose Destination
See comment in PubMed Commons below
Korean J Physiol Pharmacol. 2012 Dec;16(6):405-11. doi: 10.4196/kjpp.2012.16.6.405. Epub 2012 Dec 10.

Effects of human mesenchymal stem cell transplantation combined with polymer on functional recovery following spinal cord hemisection in rats.

Author information

  • 1Department of Physiology, Brain Korea 21 Project for Medical Science, and Brain Research Institute, Yonsei University College of Medicine, Seoul 120-752, Korea.

Abstract

The spontaneous axon regeneration of damaged neurons is limited after spinal cord injury (SCI). Recently, mesenchymal stem cell (MSC) transplantation was proposed as a potential approach for enhancing nerve regeneration that avoids the ethical issues associated with embryonic stem cell transplantation. As SCI is a complex pathological entity, the treatment of SCI requires a multipronged approach. The purpose of the present study was to investigate the functional recovery and therapeutic potential of human MSCs (hMSCs) and polymer in a spinal cord hemisection injury model. Rats were subjected to hemisection injuries and then divided into three groups. Two groups of rats underwent partial thoracic hemisection injury followed by implantation of either polymer only or polymer with hMSCs. Another hemisection-only group was used as a control. Behavioral, electrophysiological and immunohistochemical studies were performed on all rats. The functional recovery was significantly improved in the polymer with hMSC-transplanted group as compared with control at five weeks after transplantation. The results of electrophysiologic study demonstrated that the latency of somatosensory-evoked potentials (SSEPs) in the polymer with hMSC-transplanted group was significantly shorter than in the hemisection-only control group. In the results of immunohistochemical study, β-gal-positive cells were observed in the injured and adjacent sites after hMSC transplantation. Surviving hMSCs differentiated into various cell types such as neurons, astrocytes and oligodendrocytes. These data suggest that hMSC transplantation with polymer may play an important role in functional recovery and axonal regeneration after SCI, and may be a potential therapeutic strategy for SCI.

KEYWORDS:

Electrophysiology; Mesenchymal stem cells; Polymer; Spinal cord injury

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for The Korean Physiological Society and The Korean Society of Pharmacology Icon for PubMed Central
    Loading ...
    Write to the Help Desk