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J Clin Microbiol. 2013 Mar;51(3):1008-13. doi: 10.1128/JCM.02495-12. Epub 2012 Dec 26.

Multiplex real-time PCR assay for detection of methicillin-resistant Staphylococcus aureus (MRSA) strains suitable in regions of high MRSA endemicity.

Author information

1
Department of Laboratory Medicine, Gangneung Asan Hospital, University of Ulsan College of Medicine, Gangneung, South Korea. jukim@gnah.co.kr

Abstract

A multiplex real-time PCR assay that simultaneously detects the mecA, staphylococcal cassette chromosome (SCCmec)-open reading frame X (orfX) junction, and staphylococcal 16S rRNA genes was developed and evaluated using 444 staphylococcal strains. We demonstrated that this assay resulted in fewer false-positive results than a single-locus real-time PCR assay that amplified the SCCmec-orfX junction. This assay would be useful in a clinical laboratory in a region of high endemicity for methicillin-resistant Staphylococcus aureus (MRSA) infections.

PMID:
23269729
PMCID:
PMC3592057
DOI:
10.1128/JCM.02495-12
[Indexed for MEDLINE]
Free PMC Article
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