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Nat Methods. 2013 Feb;10(2):125-7. doi: 10.1038/nmeth.2301. Epub 2012 Dec 23.

Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms.

Author information

1
Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.

Abstract

We established a conditional site-specific recombination system based on dimerizable Cre recombinase-mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a new single-vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomplexan genes considered essential for host-cell invasion. Our findings uncovered the existence of an alternative invasion pathway in apicomplexan parasites.

PMID:
23263690
PMCID:
PMC3605914
DOI:
10.1038/nmeth.2301
[Indexed for MEDLINE]
Free PMC Article

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