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ACS Chem Biol. 2013 Mar 15;8(3):535-42. doi: 10.1021/cb300625g. Epub 2013 Jan 7.

HyPer-3: a genetically encoded H(2)O(2) probe with improved performance for ratiometric and fluorescence lifetime imaging.

Author information

1
Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.

Abstract

High-performance sensors for reactive oxygen species are instrumental to monitor dynamic events in cells and organisms. Here, we present HyPer-3, a genetically encoded fluorescent indicator for intracellular H2O2 exhibiting improved performance with respect to response time and speed. HyPer-3 has an expanded dynamic range compared to HyPer and significantly faster oxidation/reduction dynamics compared to HyPer-2. We demonstrate this performance by in vivo imaging of tissue-scale H2O2 gradients in zebrafish larvae. Moreover, HyPer-3 was successfully employed for single-wavelength fluorescent lifetime imaging of H2O2 levels both in vitro and in vivo.

PMID:
23256573
DOI:
10.1021/cb300625g
[Indexed for MEDLINE]

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