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Int J Infect Dis. 2013 May;17(5):e334-8. doi: 10.1016/j.ijid.2012.11.015. Epub 2012 Dec 17.

Molecular characterization of cutaneous leishmaniasis in Al-Madinah Al-Munawarah province, western Saudi Arabia.

Author information

1
Medical Laboratories Technology Department, Faculty of Applied Medical Sciences, Taibah University, Al-Madinah Al-Munawarah, PO Box 30001, Saudi Arabia. hesham_elbeshbishy@hotmail.com

Abstract

BACKGROUND:

Leishmaniasis is a parasitic disease affecting a large number of people worldwide. In this study we carried out the molecular characterization of cutaneous leishmaniasis (CL) in Al-Madinah Al-Munawarah Province, Saudi Arabia, confirming Leishmania major and Leishmania tropica as the prevalent species using molecular techniques.

METHODS:

One hundred and five patients with suspected CL were identified from four different localities in Al-Madinah Al-Munawarah Province and Al-Miqat Hospital, Al-Madinah, Saudi Arabia. Thirty-four of the 105 patients were selected at random for molecular investigation.

RESULTS:

Characterization of CL species by internal transcribed spacer 1 (ITS1) PCR-restriction fragment length polymorphism (RFLP) and kinetoplast DNA (kDNA) PCR established L. major and L. tropica as the causative organisms. kDNA PCR had a sensitivity of 90.7%, whereas ITS1 PCR had a sensitivity of 70.1%, thus facilitating the diagnosis and species identification. Parasite culture alone detected 39.2% and smear alone 55.3% of the positive samples. With the exception of kDNA PCR, all other assays were 100% specific.

CONCLUSIONS:

This study provides the first findings for the comprehensive molecular characterization of CL in Saudi Arabia.

PMID:
23253909
DOI:
10.1016/j.ijid.2012.11.015
[Indexed for MEDLINE]
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