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Anal Chem. 2013 Jan 15;85(2):816-9. doi: 10.1021/ac3032965. Epub 2012 Dec 21.

Multinozzle emitter array chips for small-volume proteomics.

Author information

1
Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Abstract

High-throughput multiplexed proteomics of small-volume biospecimens will generate new opportunities in theranostics. Achieving parallel top-down and bottom-up mass spectrometry analyses of target proteins using a unified apparatus will improve proteome characterization. We have developed a novel silicon-based microfluidic device, multinozzle emitter array chip (MEA chip), as a new platform for small-volume proteomics using liquid chromatography-nanoelectrospray ionization mass spectrometry (LC-nanoESI-MS). We demonstrate parallel, on-chip, and online LC-MS analysis of hemoglobin and its tryptic digests directly from microliters of blood, achieving a detection limit of less than 5 red blood cells. Our MEA chip will enable clinical proteomics of small-volume samples.

PMID:
23252432
PMCID:
PMC3546512
DOI:
10.1021/ac3032965
[Indexed for MEDLINE]
Free PMC Article

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