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PLoS One. 2012;7(12):e51980. doi: 10.1371/journal.pone.0051980. Epub 2012 Dec 14.

Ultra-bright and -stable red and near-infrared squaraine fluorophores for in vivo two-photon imaging.

Author information

1
Department of Cellular and Physiological Sciences and Brain Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.

Abstract

Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95.

PMID:
23251670
PMCID:
PMC3522634
DOI:
10.1371/journal.pone.0051980
[Indexed for MEDLINE]
Free PMC Article

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