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J Pediatr Hematol Oncol. 2013 Jan;35(1):14-7. doi: 10.1097/MPH.0b013e31827b12ab.

Anemia associated with acute infection in children: an animal model.

Author information

1
Department of Pediatrics Institutes of Gastroenterology, E. Wolfson Medical Center, Holon, Israel. ballin@wolfson.health.gov.il

Abstract

BACKGROUND:

We constructed an animal model to examine the possibility that erythrophagocytosis may contribute to decreased hemoglobin (Hgb) levels in acute infection in mice.

METHODS:

BALB/c mice weighing 20 to 25 g were injected (intraperitoneally) with lipopolysaccharide (LPS) (Escherichia coli serotype) of some concentrations. Control mice were injected intraperitoneally with saline (0.5 mL). Two and 4 hours after LPS administration, mice were bled (0.25 mL) for complete blood count measures and tumor necrosis factor-α and interleukin-6 levels. The mice were then killed, and their spleen, liver, and bone marrow were examined microscopically for erythrophagocytosis.

RESULTS:

After LPS administration, mouse Hgb and hematocrit levels dropped significantly. At 4 hours after LPS injection, all Hgb and hematocrit concentrations were found to be significantly lower compared with that of controls (P = 0.002 and 0.001, respectively). Significantly increased concentrations of tumor necrosis factor-α and interleukin-6 were evident after LPS injection. Prominent hepatic erythrophagocytosis was observed in the LPS-injected mice compared with controls. A significant across-group difference was observed at 4 hours, driven by significantly higher values in group 500 mcg versus controls (P = 0.005) and 100 mcg (P = 0.025). A significant increase in erythrophagocytes was observed at 2 to 4 hours in the 500 mcg LPS group (P = 0.044).

CONCLUSIONS:

Erythrophagocytosis may play a role in anemia associated with acute infection in mice.

PMID:
23249959
DOI:
10.1097/MPH.0b013e31827b12ab
[Indexed for MEDLINE]
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