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Genes Dev. 2012 Dec 15;26(24):2690-5. doi: 10.1101/gad.204602.112.

FUS binds the CTD of RNA polymerase II and regulates its phosphorylation at Ser2.

Author information

1
Howard Hughes Medical Institute.

Abstract

Mutations in the RNA-binding protein FUS (fused in sarcoma)/TLS have been shown to cause the neurodegenerative disease amyotrophic lateral sclerosis (ALS), but the normal role of FUS is incompletely understood. We found that FUS binds the C-terminal domain (CTD) of RNA polymerase II (RNAP2) and prevents inappropriate hyperphosphorylation of Ser2 in the RNAP2 CTD at thousands of human genes. The loss of FUS leads to RNAP2 accumulation at the transcription start site and a shift in mRNA isoform expression toward early polyadenylation sites. Thus, in addition to its role in alternative RNA splicing, FUS has a general function in orchestrating CTD phosphorylation during RNAP2 transcription.

PMID:
23249733
PMCID:
PMC3533074
DOI:
10.1101/gad.204602.112
[Indexed for MEDLINE]
Free PMC Article

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