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Indian J Pharmacol. 2012 Nov-Dec;44(6):732-6. doi: 10.4103/0253-7613.103273.

Shatavarins (containing Shatavarin IV) with anticancer activity from the roots of Asparagus racemosus.

Author information

1
Rajiv Gandhi University of Health Sciences, 4 T Block, Jayanagar, Bangalore, Karnataka, India.

Abstract

OBJECTIVES:

The anticancer activity of shatavarins (containing shatavarin IV) isolated from the roots of Asparagus racemosus (Wild) was evaluated using in vitro and in vivo experimental models.

MATERIAL AND METHODS:

The shatavarin IV was isolated from ethyl acetate insoluble fraction (AR-2B) of chloroform:methanol (2:1) (AR-2) extract of A. racemosus roots. The cytotoxicity (in vitro) of shatavarin IV and other shatavarins rich fraction was carried out using of MTT assay using MCF-7 (human breast cancer), HT-29 (human colon adenocarcinoma), and A-498 (human kidney carcinoma) cell lines. The in vivo anticancer activity of shatavarins (containing shatavarin IV) was evaluated against Ehrlich ascites carcinoma (EAC) tumor bearing mice.

RESULTS:

The isolated shatavarin IV (84.69 %) along with shatavarins rich fraction, coded AR-2B containing 5.05% shatavarin IV showed potent cytotoxicity. Oral administration of AR-2B to tumor bearing mice at doses of 250 and 500 mg/kg body weight for 10 days, showed significant reduction in percent increase in body weight, tumor volume, packed cell volume, viable tumor cell count, and increased non-viable cell count when compared to the untreated mice of the EAC control group. The restoration of hematological parameters towards normalcy was also observed.

CONCLUSION:

The result suggests that the shatavarins (containing shatavarin IV) rich fraction (AR-2B) exhibits significant anticancer activity in both in vitro and in vivo experimental models.

KEYWORDS:

Anticancer; Asparagus racemosus; cytotoxicity; ehrlich ascites carcinoma; shatavarin IV

PMID:
23248403
PMCID:
PMC3523501
DOI:
10.4103/0253-7613.103273
[Indexed for MEDLINE]
Free PMC Article

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