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Nat Methods. 2013 Jan;10(1):68-70. doi: 10.1038/nmeth.2287. Epub 2012 Dec 16.

Quantitative optical trapping on single organelles in cell extract.

Author information

1
Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India.

Abstract

We have developed an optical trapping method to precisely measure the force generated by motor proteins on single organelles of unknown size in cell extract. This approach, termed VMatch, permits the functional interrogation of native motor complexes. We apply VMatch to measure the force, number and activity of kinesin-1 on motile lipid droplets isolated from the liver of normally fed and food-deprived rats.

PMID:
23241632
PMCID:
PMC3605797
DOI:
10.1038/nmeth.2287
[Indexed for MEDLINE]
Free PMC Article

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