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Chem Biol Drug Des. 2013 Mar;81(3):323-33. doi: 10.1111/cbdd.12097.

Biomolecular Interactions of small-molecule inhibitors affecting the YopH protein tyrosine phosphatase.

Author information

1
United States Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA.

Abstract

We have developed competitive and direct binding methods to examine small-molecule inhibitors of protein tyrosine phosphatase activity. Focusing on the Yersinia pestis outer protein H, a potent bacterial protein tyrosine phosphatase, we describe how an understanding of the kinetic interactions involving Yersinia pestis outer protein H, peptide substrates, and small-molecule inhibitors of protein tyrosine phosphatase activity can be beneficial for inhibitor screening, and we further translate these results into a microarray assay for high-throughput screening.

PMID:
23241354
PMCID:
PMC3573263
DOI:
10.1111/cbdd.12097
[Indexed for MEDLINE]
Free PMC Article

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