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Dev Cell. 2012 Dec 11;23(6):1092-102. doi: 10.1016/j.devcel.2012.09.022.

Visualizing cell structure and function with point-localization superresolution imaging.

Author information

1
Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Abstract

Fundamental to the success of cell and developmental biology is the ability to tease apart molecular organization in cells and tissues by localizing specific proteins with respect to one another in a native cellular context. However, many key cellular structures (from mitochondrial cristae to nuclear pores) lie below the diffraction limit of visible light, precluding analysis of their organization by conventional approaches. Point-localization superresolution microscopy techniques, such as PALM and STORM, are poised to resolve, with unprecedented clarity, the organizational principles of macromolecular complexes within cells, thus leading to deeper insights into cellular function in both health and disease.

PMID:
23237943
PMCID:
PMC4221848
DOI:
10.1016/j.devcel.2012.09.022
[Indexed for MEDLINE]
Free PMC Article

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