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Wei Sheng Wu Xue Bao. 2012 Sep 4;52(9):1122-8.

[PB1-F2 protein of influenza A virus interacts with human MOAP-1 protein].

[Article in Chinese]

Author information

1
Key Laboratory of Zoonosis Ministry of Education, institute of zoonosis, Jilin University, Changchun 130062, China. cuiyuming25@163.com

Abstract

OBJECTIVE:

To identify the interaction between influenza A virus PB1-F2 and human modulator of apoptosis 1 (MOAP-1).

METHODS:

The recombinant plasmid pACT2-MOAP-1 was constructed and co-transformed into yeast AH109 with pGBKT7-PB1-F2. The growth of the co-transformants on quadruple dropout medium and beta-galactosidase activity of the reporter gene were tested. We further confirmed the interaction of cellular protein MOAP-1 and PB1-F2 by glutathione S-transferase (GST) pull-down and co-immunoprecipitation (Co-IP) assays. In addition, we investigated the effect of PB1-F2 on MOAP-1 protein level by Western blot.

RESULTS:

The results of yeast two-hybrid assay showed that MOAP-1 specifically interacted with PB1-F2 in yeast cells. Furthermore, the binding of MOAP-1 with PB1-F2 was demonstrated by glutathione S-transferase pull-down and Co-IP assays. PB1-F2 could upregulate exogenous MOAP-1 protein level.

CONCLUSION:

These results suggested that influenza virus PB1-F2 interacted with MOAP-1 and it might be involved in the regulation of cell growth and apoptosis via association with MOAP-1.

PMID:
23236846
[Indexed for MEDLINE]
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