Format

Send to

Choose Destination
See comment in PubMed Commons below
J Biomed Opt. 2013 Jan;18(1):10501. doi: 10.1117/1.JBO.18.1.010501.

Objective-free optical-resolution photoacoustic microscopy.

Author information

  • 1State University of New York, The University at Buffalo, Bio Optics and Acoustics Laboratory, Department of Biomedical Engineering, Buffalo, New York 14260, USA. chulhong@buffalo.edu

Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) becomes a premier microscopic imaging tool in biomedicine because it provides agent-free optical absorption information in tissues. By tightly focusing light to a spot, a significantly improved lateral resolution can be achieved in OR-PAM. The focal spot size is typically determined by the numerical aperture of the used objective lens. Here, we demonstrate objective-free OR-PAM using a fiber optic Bessel beam generator. In this approach, no objective lens is required and, beneficially, the complexities of conventional OR-PAM systems can be greatly relieved. We have obtained photoacoustic images of a carbon fiber with a diameter of ∼6  μm, whose lateral resolution was measured to be better than 6 to 7 μm.

PMID:
23232814
DOI:
10.1117/1.JBO.18.1.010501
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Silverchair Information Systems
    Loading ...
    Support Center