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Euro Surveill. 2012 Dec 6;17(49). pii: 20334.

Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections.

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1
Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.

Abstract

We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum.

PMID:
23231891
[Indexed for MEDLINE]
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