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Cell Oncol (Dordr). 2013 Feb;36(1):55-63. doi: 10.1007/s13402-012-0113-5. Epub 2012 Nov 15.

Expression of small nucleolar RNAs in leukemic cells.

Author information

1
Tampere Center for Child Health Research, University of Tampere School of Medicine and Tampere University Hospital, Biokatu, FIN, Tampere, Finland. kaisa.teittinen@uta.fi

Abstract

PURPOSE:

Small nucleolar RNAs (snoRNAs) direct sequence-specific modifications to ribosomal RNA. We hypothesized that the expression of snoRNAs may be altered in leukemic cells.

METHODS:

The expression of snoRNAs was analyzed in various leukemic cell lines by massive parallel sequencing (SOLiD). Quantitative real-time PCR (RT-qPCR) was used to validate the expression profiles.

RESULTS:

Our results show characteristic differences in the expression patterns of snoRNAs between cell lines representing the main subgroups of leukemia, AML, pre-B-ALL and T-ALL, respectively. In RT-qPCR analyses, several snoRNAs were found to be differentially expressed in T-ALL as compared to pre-B-ALL cell lines.

CONCLUSIONS:

snoRNAs are differentially expressed in various leukemic cell lines and could, therefore, be potentially useful in the classification of leukemia subgroups.

PMID:
23229394
DOI:
10.1007/s13402-012-0113-5
[Indexed for MEDLINE]
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