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Cancer Lett. 2013 Apr 30;331(1):58-67. doi: 10.1016/j.canlet.2012.12.001. Epub 2012 Dec 8.

CHC promotes tumor growth and angiogenesis through regulation of HIF-1α and VEGF signaling.

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1
Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 115, Taiwan.

Abstract

Pancreatic adenocarcinoma is an aggressive disease with a high mortality rate. In this study, we have newly generated a monoclonal antibody (mAb), Pa65-2, which specifically binds to pancreatic cancer cells and tumor blood vessels. The target protein of Pa65-2 is identified as human clathrin heavy chain (CHC). In vitro and In vivo study showed that suppression of CHC either by shRNA or by Pa65-2 inhibited tumor growth and angiogenesis. One of the key functions of CHC was to bind with the hypoxia-inducing factor (HIF)-1α protein, increasing the stability of this protein and facilitating its nuclear translocation, thereby regulating the expression of VEGF. Taken together, our findings indicate that CHC plays a role in the processes of tumorigenesis and angiogenesis. Pa65-2 antibody or CHC shRNA can potentially be used for pancreatic cancer therapy.

PMID:
23228632
DOI:
10.1016/j.canlet.2012.12.001
[Indexed for MEDLINE]

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