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Cell Microbiol. 2013 Jun;15(6):942-960. doi: 10.1111/cmi.12088. Epub 2013 Jan 7.

Innate immune recognition of flagellin limits systemic persistence of Brucella.

Author information

1
URBM, University of Namur (FUNDP), Belgium.
2
Department of Medical Microbiology & Immunology, University of California, Davis, CA, USA.
3
Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
4
Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA.
#
Contributed equally

Abstract

Brucella are facultative intracellular bacteria that cause chronic infections by limiting innate immune recognition. It is currently unknown whether Brucella FliC flagellin, the monomeric subunit of flagellar filament, is sensed by the host during infection. Here, we used two mutants of Brucella melitensis, either lacking or overexpressing flagellin, to show that FliC hinders bacterial replication in vivo. The use of cells and mice genetically deficient for different components of inflammasomes suggested that FliC was a target of the cytosolic innate immune receptor NLRC4 in vivo but not in macrophages in vitro where the response to FliC was nevertheless dependent on the cytosolic adaptor ASC, therefore suggesting a new pathway of cytosolic flagellin sensing. However, our work also suggested that the lack of TLR5 activity of Brucella flagellin and the regulation of its synthesis and/or delivery into host cells are both part of the stealthy strategy of Brucella towards the innate immune system. Nevertheless, as a flagellin-deficient mutant of B. melitensis wasfound to cause histologically demonstrable injuries in the spleen of infected mice, we suggested that recognition of FliC plays a role in the immunological stand-off between Brucella and its host, which is characterized by a persistent infection with limited inflammatory pathology.

PMID:
23227931
PMCID:
PMC4026035
DOI:
10.1111/cmi.12088
[Indexed for MEDLINE]
Free PMC Article
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