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PLoS One. 2012;7(11):e50738. doi: 10.1371/journal.pone.0050738. Epub 2012 Nov 30.

Optogenetic control of transcription in zebrafish.

Author information

1
National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research-Shanghai, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. htliu@sibs.ac.cn

Abstract

Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription system in yeast and zebrafish, based on the blue light dependent interaction between two plant proteins, blue light photoreceptor Cryptochrome 2 (CRY2) and the bHLH transcription factor CIB1 (CRY-interacting bHLH 1). We demonstrate the utility of this system by inducing rapid transcription suppression and activation in zebrafish.

PMID:
23226369
PMCID:
PMC3511356
DOI:
10.1371/journal.pone.0050738
[Indexed for MEDLINE]
Free PMC Article

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