Format

Send to

Choose Destination
See comment in PubMed Commons below
PLoS One. 2012;7(11):e50699. doi: 10.1371/journal.pone.0050699. Epub 2012 Nov 30.

Contribution of amino acid catabolism to the tissue specific persistence of Campylobacter jejuni in a murine colonization model.

Author information

1
Institute for Medical Microbiology, Hannover Medical School, Hannover, Germany. hofreuter.dirk@mh-hannover.de

Abstract

Campylobacter jejuni is a major cause of food-borne disease in industrialized countries. Carbohydrate utilization by C. jejuni is severely restricted, and knowledge about which substrates fuel C. jejuni infection and growth is limited. Some amino acids have been shown to serve as carbon sources both in vitro and in vivo. In the present study we investigated the contribution of serine and proline catabolism to the invitro and invivo growth of C. jejuni 81-176. We confirmed that the serine transporter SdaC and the serine ammonia-lyase SdaA are required for serine utilization, and demonstrated that a predicted proline permease PutP and a bifunctional proline/delta-1-pyrroline-5-carboxylate dehydrogenase PutA are required for proline utilization by C. jejuni 81-176. C. jejuni 81-176 mutants unable to utilize serine were shown to be severely defective for colonization of the intestine and systemic tissues in a mouse model of infection. In contrast, C. jejuni 81-176 mutants unable to utilize proline were only defective for intestinal colonization. These results further emphasize the importance of amino acid utilization in C. jejuni colonization of various tissues.

PMID:
23226358
PMCID:
PMC3511319
DOI:
10.1371/journal.pone.0050699
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Support Center