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Biomol NMR Assign. 2014 Apr;8(1):37-41. doi: 10.1007/s12104-012-9448-0. Epub 2012 Dec 8.

Sequence-specific backbone ¹H, ¹³C and ¹⁵N assignments of the catalytic domain of the Escherichia coli protein tyrosine kinase, Wzc.

Author information

1
Department of Chemistry, The City College of New York, 160 Convent Avenue, New York, NY, 10031, USA.

Abstract

Protein tyrosine kinases in bacteria are structurally and functionally distinct from their eukaryotic counterparts. The largest family of bacterial tyrosine kinases, the BY-kinase family, is highly conserved in Gram-negative and Gram-positive species, and plays a central role in biofilm and capsule formation. In Escherichia coli the BY-kinase, Wzc, is a critical component of the machinery responsible for the synthesis and export of the exo-polysaccharide colanic acid, a key constituent of biofilms. Here we present the main-chain (1)H(N), (15)N, (13)C' and (13)Cα, side-chain (13)Cβ resonance assignments for a construct that encodes the entire 274-residue cytosolic catalytic domain of Wzc.

PMID:
23225198
PMCID:
PMC3626743
DOI:
10.1007/s12104-012-9448-0
[Indexed for MEDLINE]
Free PMC Article

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