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J Biol Chem. 2013 Jan 25;288(4):2689-99. doi: 10.1074/jbc.M112.429506. Epub 2012 Dec 5.

The fidelity of transcription: RPB1 (RPO21) mutations that increase transcriptional slippage in S. cerevisiae.

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1
National Cancer Institute, Frederick National Laboratory for Cancer Research, Frederick, Maryland 21702, USA. strathej@mail.nih.gov

Abstract

The fidelity of RNA synthesis depends on both accurate template-mediated nucleotide selection and proper maintenance of register between template and RNA. Loss of register, or transcriptional slippage, is particularly likely on homopolymeric runs in the template. Transcriptional slippage can alter the coding capacity of mRNAs and is used as a regulatory mechanism. Here we describe mutations in the largest subunit of Saccharomyces cerevisiae RNA polymerase II that substantially increase the level of transcriptional slippage. Alleles of RPB1 (RPO21) with elevated slippage rates were identified among 6-azauracil-sensitive mutants and were also isolated using a slippage-dependent reporter gene. Biochemical characterization of polymerase II isolated from these mutants confirms elevated levels of transcriptional slippage.

PMID:
23223234
PMCID:
PMC3554935
DOI:
10.1074/jbc.M112.429506
[Indexed for MEDLINE]
Free PMC Article
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