Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2013 Jan;10(1):60-3. doi: 10.1038/nmeth.2277. Epub 2012 Dec 9.

Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.

Author information

1
Joint Graduate Group in Bioengineering, University of California, San Francisco/University of California, Berkeley, San Francisco, California, USA. sara.abrahamsson@gmail.com

Abstract

Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.

PMID:
23223154
PMCID:
PMC4161287
DOI:
10.1038/nmeth.2277
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center