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Plant Signal Behav. 2013 Feb;8(2):e23021. doi: 10.4161/psb.23021. Epub 2012 Dec 6.

Genome-wide analysis of glutathione reductase (GR) genes from rice and Arabidopsis.

Author information

1
Plant Molecular Biology Group; International Centre for Genetic Engineering and Biotechnology; Aruna Asaf Ali Marg; New Delhi, India.
2
Stress Physiology and Molecular Biology Lab; Centre for Biotechnology; MD University; Rohtak, India.

Abstract

Plant cells and tissues remain always on risk under abiotic and biotic stresses due to increased production of reactive oxygen species (ROS). Plants protect themselves against ROS induced oxidative damage by the upregulation of antioxidant machinery. Out of many components of antioxidant machinery, glutathione reductase (GR, EC 1.6.4.2) and glutathione (GSH, γ-Glu-Cys-Gly) play important role in the protection of cell against oxidative damage. In stress condition, the GR helps in maintaining the reduced glutathione pool for strengthening the antioxidative processes in plants. Present study investigates genome wide analysis of GR from rice and Arabidopsis. We were able to identify 3 rice GR genes (LOC_Os02 g56850, LOC_Os03 g06740, LOC_Os10 g28000) and 2 Arabidopsis GR genes (AT3G54660, AT3G24170) from their respective genomes on the basis of their annotation as well as the presence of pyridine nucleotide-disulphide oxidoreductases class-I active site. The evolutionary relationship of the GR genes from rice and Arabidopsis genomes was analyzed using the multiple sequence alignment and phylogenetic tree. This revealed evolutionary conserved pyridine nucleotide-disulphide oxidoreductases class-I active site among the GR protein in rice and Arabidopsis. This study should make an important contribution to our better understanding of the GR under normal and stress condition in plants.

KEYWORDS:

Arabidopsis; abiotic stress; chromosome localization; genome-wide analysis; glutathione reductase; homology modeling; phylogenetic analysis; rice

PMID:
23221779
PMCID:
PMC3657001
DOI:
10.4161/psb.23021
[Indexed for MEDLINE]
Free PMC Article

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