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Biosci Biotechnol Biochem. 2012;76(12):2354-6. Epub 2012 Dec 7.

Toxoflavin lyase enzyme as a marker for selecting potato plant transformants.

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1
Green-Bio Research Center, KRIBB, Daejeon, Korea.

Abstract

This study established a new system for potato transformation using toxoflavin as selection agent and toxoflavin lyase (tflA) as selectable marker gene. Potato plants expressing tflA was successfully transformed on toxoflavin medium with 27% efficiency, similar to that for the hygromycin/hpt selection system. The transgenic potato expressing tflA also showed resistance to Burkholderia glumea infection.

PMID:
23221711
DOI:
10.1271/bbb.120593
[Indexed for MEDLINE]
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