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Biosci Biotechnol Biochem. 2012;76(12):2335-7. Epub 2012 Dec 7.

A distinct binding mode of archaeal ribonuclease P proteins to RNA.

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1
Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.

Abstract

The ribonuclease P (RNase P) in the hyperthermophilic archaeon Pyrococcus horikoshii comprises RNA (PhopRNA) and five proteins. We analyzed the RNA binding mode of the protein, using a pair of complementary fluorescence-labeled oligoribonucleotides. Fluorescence resonance energy transfer (FRET)-based assays suggested that the RNase P proteins assist PhopRNA in attaining a functionally active conformation via a distinct mode of binding.

PMID:
23221704
DOI:
10.1271/bbb.120546
[Indexed for MEDLINE]
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