Format

Send to

Choose Destination
See comment in PubMed Commons below
Virus Res. 2013 Jan;171(1):257-61. doi: 10.1016/j.virusres.2012.11.014. Epub 2012 Dec 5.

RNA helicase is involved in the expression and replication of classical swine fever virus and interacts with untranslated region.

Author information

1
College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China.

Abstract

To investigate whether cytoplasmic RNA helicase A (RHA) influences the expression and replication of classical swine fever virus (CSFV), an siRNA molecule targeted to RHA was transfected into PK-15 cells. The siRNA was found to reduce cytoplasmic RHA. In CSFV subgenomic replicon transfected cells, incubation with the siRNAs negatively impacted viral NS3 and RNA production. In the CSFV infected cells, treatment with the siRNA resulted in a significant reduction of viral replication by 65-70%. Furthermore, affinity chromatography and UV-crosslinking assays revealed that RHA can bind the 5' and 3' terminal region of CSFV 3'-untranslated region (UTR), the 5' terminal region and domain III of CSFV 5' UTR. All these regions are important for viral replication and translation. These data showed that RHA is involved in the expression and replication of CSFV and might participate in modulation of RNA synthesis, replication and translation of CSFV by binding these regions.

PMID:
23220337
DOI:
10.1016/j.virusres.2012.11.014
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center