Format

Send to

Choose Destination
See comment in PubMed Commons below
Phys Rev Lett. 2012 Oct 19;109(16):168102. Epub 2012 Oct 17.

Unified resolution bounds for conventional and stochastic localization fluorescence microscopy.

Author information

1
Department of Physics, Stanford University, Stanford, California 94305, USA. eran@post.harvard.edu

Abstract

Superresolution microscopy enables imaging in the optical far field with ~20 nm-scale resolution. However, classical concepts of resolution using point-spread and modulation-transfer functions fail to describe the physical limits of superresolution techniques based on stochastic localization of single emitters. Prior treatments of stochastic localization microscopy have defined how accurately a single emitter's position can be determined, but these bounds are restricted to sparse emitters, do not describe conventional microscopy, and fail to provide unified concepts of resolution for all optical methods. Here we introduce a measure of resolution, the information transfer function (ITF), that gives physical limits for conventional and stochastic localization techniques. The ITF bounds the accuracy of image determination as a function of spatial frequency and for conventional microscopy is proportional to the square of the modulation-transfer function. We use the ITF to describe how emitter density and photon counts affect imaging performance across the continuum from conventional to superresolution microscopy, without assuming emitters are sparse. This unified physical description of resolution facilitates experimental choices and designs of image reconstruction algorithms.

PMID:
23215134
PMCID:
PMC3521605
DOI:
10.1103/PhysRevLett.109.168102
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Physical Society Icon for PubMed Central
    Loading ...
    Support Center