Format

Send to

Choose Destination
See comment in PubMed Commons below
Biochem Biophys Res Commun. 2013 Jan 11;430(2):729-34. doi: 10.1016/j.bbrc.2012.11.081. Epub 2012 Dec 1.

Co-culture with endothelial progenitor cells promotes survival, migration, and differentiation of osteoclast precursors.

Author information

1
Department of Orthopaedics, Southwest Hospital, The Third Military Medical University, Chongqing, China.

Abstract

In this study, we report the effect of endothelial progenitor cells (EPCs) on the biological behavior of osteoclast precursors in vitro by establishing an indirect co-culture system of mice EPCs and RAW 264.7 monocyte cells. Results show that the survival, migration, and differentiation of osteoclast precursors were greatly enhanced when co-cultured with EPCs. These phenotypic changes coincide with the upregulation of multiple genes affected cell behavior, including phospho-VEGFR-2, CXCR4, phospho-Smad2/3, phospho-Akt, phospho-ERK1, and phospho-p38 MAPK. The results collectively suggest that EPCs could modulate the survival, migration, and differentiation potential of osteoclast precursors, thus providing new insights in understanding of correlation between angiogenesis and bone homeostasis.

PMID:
23206710
DOI:
10.1016/j.bbrc.2012.11.081
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center