Format

Send to

Choose Destination
Biochem Biophys Res Commun. 2013 Jan 11;430(2):763-8. doi: 10.1016/j.bbrc.2012.11.074. Epub 2012 Dec 1.

Novel application of stem cell-derived factors for periodontal regeneration.

Author information

1
Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Japan. t-inukai@med.nagoya-u.ac.jp

Abstract

The effect of conditioned medium from cultured mesenchymal stem cells (MSC-CM) on periodontal regeneration was evaluated. In vitro, MSC-CM stimulated migration and proliferation of dog MSCs (dMSCs) and dog periodontal ligament cells (dPDLCs). Cytokines such as insulin-like growth factor, vascular endothelial growth factor, transforming growth factor-β1, and hepatocyte growth factor were detected in MSC-CM. In vivo, one-wall critical-size, intrabony periodontal defects were surgically created in the mandible of dogs. Dogs with these defects were divided into three groups that received MSC-CM, PBS, or no implants. Absorbable atelo-collagen sponges (TERUPLUG®) were used as a scaffold material. Based on radiographic and histological observation 4 weeks after transplantation, the defect sites in the MSC-CM group displayed significantly greater alveolar bone and cementum regeneration than the other groups. These findings suggest that MSC-CM enhanced periodontal regeneration due to multiple cytokines contained in MSC-CM.

PMID:
23206704
DOI:
10.1016/j.bbrc.2012.11.074
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center