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Adv Healthc Mater. 2013 May;2(5):718-27. doi: 10.1002/adhm.201200335. Epub 2012 Nov 30.

Antibody-functionalized porous silicon nanoparticles for vectorization of hydrophobic drugs.

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1
Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-ENSCM-UM2-UM1, Ecole Nationale Supérieure de Chimie de Montpellier, France.

Abstract

We describe the preparation of biodegradable porous silicon nanoparticles (pSiNP) functionalized with cancer cell targeting antibodies and loaded with the hydrophobic anti-cancer drug camptothecin. Orientated immobilization of the antibody on the pSiNP is achieved using novel semicarbazide based bioconjugate chemistry. To demonstrate the generality of this targeting approach, the three antibodies MLR2, mAb528 and Rituximab are used, which target neuroblastoma, glioblastoma and B lymphoma cells, respectively. Successful targeting is demonstrated by means of flow cytometry and immunocytochemistry both with cell lines and primary cells. Cell viability assays after incubation with pSiNPs show selective killing of cells expressing the receptor corresponding to the antibody attached on the pSiNP.

PMID:
23203914
DOI:
10.1002/adhm.201200335
[Indexed for MEDLINE]
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