Multiplex protein detection with DNA readout via mass spectrometry

N Biotechnol. 2013 Jan 25;30(2):153-8. doi: 10.1016/j.nbt.2012.11.003. Epub 2012 Nov 28.

Abstract

Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody-oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens
  • DNA / analysis*
  • Polymerase Chain Reaction
  • Proteins / analysis*
  • Proteins / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Proteins
  • DNA