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Cell Rep. 2012 Dec 27;2(6):1537-45. doi: 10.1016/j.celrep.2012.10.023. Epub 2012 Nov 29.

Specific miRNA stabilization by Gld2-catalyzed monoadenylation.

Author information

1
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.

Abstract

MicroRNAs (miRNAs) are small, noncoding RNAs that inhibit translation and promote mRNA decay. The levels of mature miRNAs are the result of different rates of transcription, processing, and turnover. The noncanonical polymerase Gld2 has been implicated in the stabilization of miR-122, possibly through catalyzing 3' monoadenylation; however, there is little evidence that this relationship is one of cause and effect. Here, we biochemically characterize Gld2's involvement in miRNA monoadenylation and its effect on miRNA stability. We find that Gld2 directly monoadenylates and stabilizes specific miRNA populations in human fibroblasts and that sensitivity to monoadenylation-induced stability depends on nucleotides in the miRNA 3' end. These results establish a mechanism of miRNA stability and resulting posttranscriptional gene regulation.

PMID:
23200856
PMCID:
PMC3534910
DOI:
10.1016/j.celrep.2012.10.023
[Indexed for MEDLINE]
Free PMC Article

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