Format

Send to

Choose Destination
See comment in PubMed Commons below
Curr Opin Cell Biol. 2012 Dec;24(6):793-801. doi: 10.1016/j.ceb.2012.11.001. Epub 2012 Nov 27.

Pluripotency in 3D: genome organization in pluripotent cells.

Author information

1
Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, Molecular Biology Institute, Department of Biological Chemistry, David Geffen School of Medicine of the University of California, Los Angeles, CA, United States.

Abstract

Cells face the challenge of storing two meters of DNA in the three-dimensional (3D) space of the nucleus that spans only a few microns. The nuclear organization that is required to overcome this challenge must allow for the accessibility of the gene regulatory machinery to the DNA and, in the case of embryonic stem cells (ESCs), for the transcriptional and epigenetic changes that accompany differentiation. Recent technological advances have allowed for the mapping of genome organization at an unprecedented resolution and scale. These breakthroughs have led to a deluge of new data, and a sophisticated understanding of the relationship between gene regulation and 3D genome organization is beginning to form. In this review we summarize some of the recent findings illuminating the 3D structure of the eukaryotic genome, as well as the relationship between genome topology and function from the level of whole chromosomes to enhancer-promoter loops with a focus on features affecting genome organization in ESCs and changes in nuclear organization during differentiation.

PMID:
23199754
PMCID:
PMC3540188
DOI:
10.1016/j.ceb.2012.11.001
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center