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Med Sci Monit. 2012 Dec;18(12):BR469-74.

Excess dietary methionine does not affect fracture healing in mice.

Author information

1
Department of Trauma, Hand and Reconstructive Surgery, University of Saarland, Homburg/Saar, Germany. joerg.holstein@uks.eu

Abstract

BACKGROUND:

An elevated serum concentration of homocysteine (hyperhomocysteinemia) has been shown to disturb fracture healing. As the essential amino acid, methionine, is a precursor of homocysteine, we aimed to investigate whether excess methionine intake affects bone repair.

MATERIAL/METHODS:

We analyzed bone repair in 2 groups of mice. One group was fed a methionine-rich diet (n=13), and the second group received an equicaloric control diet without methionine supplementation (n=12). Using a closed femoral fracture model, bone repair was analyzed by histomorphometry and biomechanical testing at 4 weeks after fracture. Blood was sampled to measure serum concentrations of homocysteine, the bone formation marker osteocalcin, and the bone resorption marker collagen I C-terminal crosslaps

RESULTS:

Serum concentrations of homocysteine were significantly higher in the methionine group than in the control group, while serum markers of bone turnover did not differ significantly between the 2 groups. Histomorphometry revealed no significant differences in size and tissue composition of the callus between animals fed the methionine-enriched diet and those receiving the control diet. Accordingly, animals of the 2 groups showed a comparable bending stiffness of the healing bones.

CONCLUSIONS:

We conclude that excess methionine intake causes hyperhomocysteinemia, but does not affect fracture healing in mice.

PMID:
23197225
PMCID:
PMC3560796
[Indexed for MEDLINE]
Free PMC Article
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