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Int J Radiat Oncol Biol Phys. 2013 May 1;86(1):128-35. doi: 10.1016/j.ijrobp.2012.10.019. Epub 2012 Nov 27.

Lung irradiation increases mortality after influenza A virus challenge occurring late after exposure.

Author information

1
Department of Environmental Medicine, University of Rochester Medical Center, Rochester, New York 14642, USA.

Abstract

PURPOSE:

To address whether irradiation-induced changes in the lung environment alter responses to a viral challenge delivered late after exposure but before the appearance of late lung radiation injury.

METHODS AND MATERIALS:

C57BL/6J mice received either lung alone or combined lung and whole-body irradiation (0-15 Gy). At 10 weeks after irradiation, animals were infected with 120 HAU influenza virus strain A/HKx31. Innate and adaptive immune cell recruitment was determined using flow cytometry. Cytokine and chemokine production and protein leakage into the lung after infection were assessed.

RESULTS:

Prior irradiation led to a dose-dependent failure to regain body weight after infection and exacerbated mortality, but it did not affect virus-specific immune responses or virus clearance. Surviving irradiated animals displayed a persistent increase in total protein in bronchoalveolar lavage fluid and edema.

CONCLUSIONS:

Lung irradiation increased susceptibility to death after infection with influenza virus and impaired the ability to complete recovery. This altered response does not seem to be due to a radiation effect on the immune response, but it may possibly be an effect on epithelial repair.

PMID:
23195776
PMCID:
PMC3608839
DOI:
10.1016/j.ijrobp.2012.10.019
[Indexed for MEDLINE]
Free PMC Article
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