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Food Chem. 2013 Feb 15;136(3-4):1478-83. doi: 10.1016/j.foodchem.2012.09.047. Epub 2012 Sep 21.

Nanocolloidal gold-based immuno-dip strip assay for rapid detection of Sudan red I in food samples.

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College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.


A semiquantitative dip strip assay was developed using nanocolloidal gold-labelled monoclonal antibody (Mab) 8A10 for the rapid detection of Sudan red I in food samples. A protein-Sudan red I conjugate was coated on a nitro cellulose membrane strip in a defined test line. In flow of the complex of nanocolloidal gold labelled-Mab and Sudan red I along the strip, intensive red colour that was formed in the test line reflected the Sudan red I concentration. The test required 10 min and had a visual limit of detection of 10 ng/g Sudan red I in tomato sauce and chilli powder samples. The results of the strip assay agreed well with those of a high performance liquid chromatography method for both spiked and real commercial samples. The strip was stable for at least 2 months at 4°C. The strip assay offers the potential as a useful rapid and simple method for screening of Sudan red I in food samples.

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