Organizational differences can be detected despite differences in cell size and shape between different populations. (*A*) Network diagram of the comparisons used in generating the graph to the right. Every simulation is compared with the “Rand sim” point placement in cc124. (*B*) Spatial bias can be detected despite variation in cell size, shape, and organelle number between mutant and WT strains. Statistically significant differences are seen between all compared groups, with the exception of the random-to-random comparisons, which are not statistically significant. The ellipse configuration and number of organelles from each of the real cells were used to generate synthetic datasets (corresponding directly to each real population) using the nonbiased and biased simulation methods of . The distance between the synthetic cc124 dataset generated with a random distribution of points and each of the synthetic datasets was calculated. Simulations of uniform point distribution in both population pairs (dark red) contrast strongly with simulations using uniform distribution in one population and biased distribution in the other, revealing sensitivity to changes in organizational type despite variation in cell size, shape, or organelle number. Dist, distance; Max, maximum; Min, minimum; Rand, random; Sim, simulation.

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