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Indian J Med Microbiol. 2012 Oct-Dec;30(4):403-6. doi: 10.4103/0255-0857.103759.

Comparison of HIV-1 RNA level estimated with plasma and DBS samples: a pilot study from India (South).

Author information

1
Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu-632004, India.

Abstract

PURPOSE:

The use of dried blood spots (DBS) for HIV-1 viral load determination could greatly enhance the management of HIV infected individuals in resource-limited countries.

OBJECTIVE:

To compare the HIV-1 viral load values obtained between parallel collected plasma and DBS.

MATERIALS AND METHODS:

DBS and plasma samples were collected from 62 HIV-1 infected individuals and were used for determination of HIV-1 RNA concentrations using the Abbot real-time HIV-1 PCR.

RESULT:

Mean of the log difference of viral load values between plasma and DBS was -0.41 log. DBS viral load values significantly correlated with plasma viral load (r = 0.9818, P < 0.0001).

CONCLUSION:

These results suggest that DBS samples can be used as an alternative to plasma for the estimation of HIV-1 viral load if samples are appropriately stored.

PMID:
23183463
DOI:
10.4103/0255-0857.103759
[Indexed for MEDLINE]
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