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Bioresour Technol. 2013 May;135:459-63. doi: 10.1016/j.biortech.2012.10.042. Epub 2012 Oct 22.

Improved acid tolerance of Lactobacillus pentosus by error-prone whole genome amplification.

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Institute of Chemical and Engineering Sciences, Agency for Sciences, Technology and Research (A*STAR), 1 Pesek Road, Jurong Island, Singapore 627833, Singapore.


Acid tolerance of Lactobacillus pentosus ATCC 8041 was improved by error-prone amplification of its genomic DNA using random primers and Taq DNA polymerase. The resulting amplification products were transferred into wild-type L. pentosus by electroporation and the transformants were screened for growth on low-pH agar plates. After only one round of mutation, one mutant (MT3) was identified that was able to completely consume 20 g/L of glucose to produce lactic acid at a yield of 95% in 1L MRS medium at pH 3.8 within 36 h, whereas no growth or lactic acid production was observed for the wild-type strain under the same conditions. The acid tolerance of mutant MT3 remained genetically stable for at least 25 subcultures. Therefore, the error-prone whole genome amplification technique is a very powerful tool for improving phenotypes of this lactic acid bacterium and may also be applicable for other microorganisms.

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