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Nanomedicine. 2013 Jul;9(5):636-45. doi: 10.1016/j.nano.2012.10.011. Epub 2012 Nov 22.

Cellular biophysical dynamics and ion channel activities detected by AFM-based nanorobotic manipulator in insulinoma β-cells.

Author information

1
College of Engineering, Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, USA.

Abstract

Distinct biochemical, electrochemical and electromechanical coupling processes of pancreatic β-cells may well underlie different response patterns of insulin release from glucose and capsaicin stimulation. Intracellular Ca(2+) levels increased rapidly and dose-dependently upon glucose stimulation, accompanied with about threefold rapid increases in cellular stiffness. Subsequently, cellular stiffness diminished rapidly and settled at a value about twofold of the baseline. Capsaicin caused a similar transient increase in intracellular Ca(2+) changes. However, cellular stiffness increased gradually to about twofold until leveling off. The current study characterizes for the first time the biophysical properties underlying glucose-induced biphasic responses of insulin secretion, distinctive from the slow and single-phased stiffness response to capsaicin despite similar changes in intracellular Ca(2+) levels. The integrated AFM nanorobotics and optical investigation enables the fine dissection of mechano-property from ion channel activities in response to specific and non-specific agonist stimulation, providing novel biomechanical markers for the insulin secretion process.

FROM THE CLINICAL EDITOR:

This study characterizes the biophysical properties underlying glucose-induced biphasic responses of insulin secretion. Integrated AFM nanorobotics and optical investigations provided novel biomechanical markers for the insulin secretion process.

PMID:
23178285
PMCID:
PMC3594338
DOI:
10.1016/j.nano.2012.10.011
[Indexed for MEDLINE]
Free PMC Article

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