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Mol Cell. 2013 Jan 10;49(1):109-20. doi: 10.1016/j.molcel.2012.10.017. Epub 2012 Nov 21.

Mitosis-specific regulation of nuclear transport by the spindle assembly checkpoint protein Mad1p.

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1
Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.

Abstract

Nuclear pore complexes (NPCs) and kinetochores perform distinct tasks, yet their shared ability to bind several proteins suggests their functions are intertwined. Among these shared proteins is Mad1p, a component of the yeast spindle assembly checkpoint (SAC). Here we describe a role for Mad1p in regulating nuclear import that employs its ability to sense a disruption of kinetochore-microtubule interactions during mitosis. We show that kinetochore-microtubule detachment arrests nuclear import mediated by the transport factor Kap121p through a mechanism that requires Mad1p cycling between unattached, metaphase kinetochores and binding sites at the NPC. This signaling pathway requires the Aurora B-like kinase Ipl1p, and the resulting transport changes inhibit the nuclear import of Glc7p, a phosphatase that acts as an Ipl1p antagonist. We propose that a distinct branch of the SAC exists in which Mad1p senses unattached kinetochores and, by altering NPC transport activity, regulates the nuclear environment of the spindle.

PMID:
23177738
DOI:
10.1016/j.molcel.2012.10.017
[Indexed for MEDLINE]
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