Format

Send to

Choose Destination
J Proteome Res. 2013 Jan 4;12(1):199-207. doi: 10.1021/pr300758c. Epub 2012 Dec 12.

A periodate-cleavable linker for functional proteomics under slightly acidic conditions: application for the analysis of intracellular aspartic proteases.

Author information

1
Interfaculty Institute of Biochemistry, University of Tuebingen, Ob dem Himmelreich 7, 72074 Tuebingen, Germany.

Abstract

Specific elution of captured proteins greatly improves the quality of proteomic data obtained from pull-downs by avoiding signals from nonspecific proteins, thus allowing more sensitive identification of target proteins. This is important in activity-based proteomics or drug target identification. However, commonly used chemically cleavable linkers can only be cleaved at close to neutral pH, which prevents them from being used for proteins binding only at lower pH when no cross-linking is applied. On the other hand, elution of common acid-cleavable labels can also coelute proteins bound by ionic interactions. Here, we report the synthesis and application of a label readily cleavable by mild oxidation at moderately acidic pH for the noncovalent labeling and pull-down of intracellular aspartic proteases. Using specific release, target proteins cathepsin D and napsin A were identified from human kidney with much higher confidence and without any nontarget hits.

PMID:
23176076
DOI:
10.1021/pr300758c
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center