Format

Send to

Choose Destination
See comment in PubMed Commons below
Development. 2013 Jan 1;140(1):56-65. doi: 10.1242/dev.086413. Epub 2012 Nov 22.

Hedgehog is required for CySC self-renewal but does not contribute to the GSC niche in the Drosophila testis.

Author information

1
Department of Biochemistry and Molecular Biology, New York University School of Medicine, New York, NY 10016, USA.

Abstract

The Drosophila testis harbors two types of stem cells: germ line stem cells (GSCs) and cyst stem cells (CySCs). Both stem cell types share a physical niche called the hub, located at the apical tip of the testis. The niche produces the JAK/STAT ligand Unpaired (Upd) and BMPs to maintain CySCs and GSCs, respectively. However, GSCs also require BMPs produced by CySCs, and as such CySCs are part of the niche for GSCs. Here we describe a role for another secreted ligand, Hedgehog (Hh), produced by niche cells, in the self-renewal of CySCs. Hh signaling cell-autonomously regulates CySC number and maintenance. The Hh and JAK/STAT pathways act independently and non-redundantly in CySC self-renewal. Finally, Hh signaling does not contribute to the niche function of CySCs, as Hh-sustained CySCs are unable to maintain GSCs in the absence of Stat92E. Therefore, the extended niche function of CySCs is solely attributable to JAK/STAT pathway function.

PMID:
23175633
PMCID:
PMC3513992
DOI:
10.1242/dev.086413
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center