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J Clin Microbiol. 2013 Feb;51(2):615-7. doi: 10.1128/JCM.03009-12. Epub 2012 Nov 21.

Detection of heterogeneity of Borrelia burgdorferi in ixodes ticks by culture-dependent and culture-independent methods.

Author information

1
Laboratory of Medical Zoology, Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA.

Abstract

Abilities to detect heterogeneity of ospC genotypes of the Lyme disease spirochete Borrelia burgdorferi in the tick vector by in vitro culture (IVC) and direct PCR (dPCR) were compared. IVC failed to detect one-third of the ospC genotypes detected by dPCR. Among IVC results, common ospC genotypes were overrepresented while occurrence of rare genotypes was underestimated.

PMID:
23175266
PMCID:
PMC3553859
DOI:
10.1128/JCM.03009-12
[Indexed for MEDLINE]
Free PMC Article

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