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G3 (Bethesda). 2012 Nov;2(11):1369-77. doi: 10.1534/g3.112.003723. Epub 2012 Nov 1.

A germline clone screen on the X chromosome reveals novel meiotic mutants in Drosophila melanogaster.

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Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, MO 64110, USA.


In an effort to isolate novel meiotic mutants that are severely defective in chromosome segregation and/or exchange, we employed a germline clone screen of the X chromosome of Drosophila melanogaster. We screened over 120,000 EMS-mutagenized chromosomes and isolated 19 mutants, which comprised nine complementation groups. Four of these complementation groups mapped to known meiotic genes, including mei-217, mei-218, mei-9, and nod. Importantly, we have identified two novel complementation groups with strong meiotic phenotypes, as assayed by X chromosome nondisjunction. One complementation group is defined by three alleles, and the second novel complementation group is defined by a single allele. All 19 mutants are homozygous viable, fertile, and fully recessive. Of the 9 mutants that have been molecularly characterized, 5 are canonical EMS-induced transitions, and the remaining 4 are transversions. In sum, we have identified two new genes that are defined by novel meiotic mutants, in addition to isolating new alleles of mei-217, mei-218, mei-9, and nod.


chromosome segregation; meiosis; mutant screens

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