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G3 (Bethesda). 2012 Nov;2(11):1369-77. doi: 10.1534/g3.112.003723. Epub 2012 Nov 1.

A germline clone screen on the X chromosome reveals novel meiotic mutants in Drosophila melanogaster.

Author information

1
Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, MO 64110, USA.

Abstract

In an effort to isolate novel meiotic mutants that are severely defective in chromosome segregation and/or exchange, we employed a germline clone screen of the X chromosome of Drosophila melanogaster. We screened over 120,000 EMS-mutagenized chromosomes and isolated 19 mutants, which comprised nine complementation groups. Four of these complementation groups mapped to known meiotic genes, including mei-217, mei-218, mei-9, and nod. Importantly, we have identified two novel complementation groups with strong meiotic phenotypes, as assayed by X chromosome nondisjunction. One complementation group is defined by three alleles, and the second novel complementation group is defined by a single allele. All 19 mutants are homozygous viable, fertile, and fully recessive. Of the 9 mutants that have been molecularly characterized, 5 are canonical EMS-induced transitions, and the remaining 4 are transversions. In sum, we have identified two new genes that are defined by novel meiotic mutants, in addition to isolating new alleles of mei-217, mei-218, mei-9, and nod.

KEYWORDS:

chromosome segregation; meiosis; mutant screens

PMID:
23173088
PMCID:
PMC3484667
DOI:
10.1534/g3.112.003723
[Indexed for MEDLINE]
Free PMC Article
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