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Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):20566-71. doi: 10.1073/pnas.1206970109. Epub 2012 Nov 20.

Low-affinity B cells transport viral particles from the lung to the spleen to initiate antibody responses.

Author information

1
Research Department, Cytos Biotechnology, CH-8952 Zurich-Schlieren, Switzerland.

Abstract

The lung is an important entry site for pathogens; its exposure to antigens results in systemic as well as local IgA and IgG antibodies. Here we show that intranasal administration of virus-like particles (VLPs) results in splenic B-cell responses with strong local germinal-center formation. Surprisingly, VLPs were not transported from the lung to the spleen in a free form but by B cells. The interaction between VLPs and B cells was initiated in the lung and occurred independently of complement receptor 2 and Fcγ receptors, but was dependent upon B-cell receptors. Thus, B cells passing through the lungs bind VLPs via their B-cell receptors and deliver them to local B cells within the splenic B-cell follicle. This process is fundamentally different from delivery of blood or lymph borne particulate antigens, which are transported into B cell follicles by binding to complement receptors on B cells.

PMID:
23169669
PMCID:
PMC3528531
DOI:
10.1073/pnas.1206970109
[Indexed for MEDLINE]
Free PMC Article
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